Goat anti-Rat IgG2ab, Subclass Specific Antibody (HRP)

Catalog No. ABIN568675

Product Details

Target: IgG2ab (IgG2ab, Subclass Specific)

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Western Blotting (WB)

Characteristics: Molar Ratio: Peroxidase/IgG aprox. 1.0

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.

Immunogen: Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1,0 mL distilled water. Working dilutions should be prepared by adding sterile PBS, pH 7.2

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -28 °C for longer.

Target Details

Target: IgG2ab (IgG2ab, Subclass Specific)

Alternative Name: IgG2ab (IgG2ab Products)

Background: Synonyms: Rat Immunoglobulin G2ab