Rabbit anti-Mouse lambda (Light Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN5608187

Product Details

Target: lambda

Binding Specificity: Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse lambda light chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: lambda

Alternative Name: lambda )

Background:

Synonyms: Rabbit Anti-Mouse λ (lambda chain) Biotin Conjugated Antibody, Rabbit Anti Mouse lambda Antibody Biotin conjugation

Background: Anti-Mouse λ (lambda chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse lambda light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG lambda is conjugated to biotin.