Donkey anti-Human IgM (Fc5mu Region) Antibody (PE) - Preadsorbed

Catalog No. ABIN5608181

Product Details

Target: IgM

Fragment: F(ab')2 fragment

Binding Specificity: Fc5mu Region

Reactivity: Human

Host: Donkey

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgM Fc5mu  fragment

Isotype: IgG

Application Details

Application Notes:

Application Note: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.

Expiry Date: 6 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: Donkey F(ab')2 anti-Human IgM Fc5u Antibody Phycoerythrin Conjugation, Donkey F(ab')2 anti-Human IgM Fc5u PE Conjugated Antibody

Background: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody generated in donkey detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.