Rabbit anti-Mouse IgA (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN5608174

Product Details

Target: IgA

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgA alpha heavy chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Monkey IgA antibody is suitable for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for other applications should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Judd, Bredin, Kalantzis, Jenkins, Ernst, Giraud: "STAT3 activation regulates growth, inflammation, and vascularization in a mouse model of gastric tumorigenesis." in: Gastroenterology, Vol. 131, Issue 4, pp. 1073-85, (2006) (PubMed).

Howlett, Judd, Jenkins, La Gruta, Grail, Ernst, Giraud: "Differential regulation of gastric tumor growth by cytokines that signal exclusively through the coreceptor gp130." in: Gastroenterology, Vol. 129, Issue 3, pp. 1005-18, (2005) (PubMed).

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti-Mouse IgA (alpha chain) biotin conjugated Antibody, Rabbit Anti-Mouse IgA alpha chain Antibody biotin conjugation

Background: Anti-Monkey IgA Antibody generated in rabbit detects immunoglobulin A alpha chain from monkey. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Monkey IgA is conjugated to biotin.