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Rabbit anti-Mouse lambda (Light Chain) Antibody (TRITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG TRITC
Catalog No. ABIN5608170
  • Target
    lambda
    Binding Specificity
    • 18
    • 10
    Light Chain
    Reactivity
    • 27
    • 13
    • 4
    Mouse
    Host
    • 26
    • 8
    • 6
    • 4
    Rabbit
    Clonality
    • 32
    • 12
    Polyclonal
    Conjugate
    • 8
    • 8
    • 8
    • 7
    • 4
    • 3
    • 3
    • 3
    TRITC
    Application
    • 32
    • 32
    • 12
    • 12
    • 8
    • 8
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Characteristics
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse lambda light chain

    Isotype
    IgG
    Labeling Ratio
    3.2
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    lambda
    Alternative Name
    lambda )
    Background

    Synonyms: Rabbit Anti-Mouse λ (lambda chain) rhodamine Conjugated Antibody, Rabbit Anti-Mouse lambda Antibody TRITC conjugation

    Background: Anti-Mouse λ (lambda chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse lambda light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG lambda is conjugated to Rhodamine.

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