Goat anti-Cow IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN5608132

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Bovine IgG (H&L) Antibody Peroxidase Conjugated detects Bovine Immunoglobulin G. Immunoglobulin G, IgG, are antibody molecules. Each IgG is a hetereotetramer composed of four peptide chains - two heavy chains and two light chains. Each IgG has two F(ab) antigen binding sites.

Purification: Anti-Bovine IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Bovine IgG and Bovine Serum.

Immunogen:

Immunogen: Bovine IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:5,000

Application Note: Anti-Bovine IgG (heavy and light chain) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to bovine immunoglobulin is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.

ELISA Dilution: 1:5,000 - 1:100,000

Western Blot Dilution: 1:10,000 - 1:200,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.8 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: None. Do NOT add sodium azide!

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Bovine IgG peroxidase Conjugated Antibody, goat anti-Bovine IgG HRP Conjugated Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Bovine IgG (H&L) Antibody Peroxidase Conjugated detects Bovine Immunoglobulin G. Immunoglobulin G, IgG, are antibody molecules. Each IgG is a hetereotetramer composed of four peptide chains - two heavy chains and two light chains. Each IgG has two F(ab) antigen binding sites.