Goat anti-Human IgA Antibody (HRP)

Catalog No. ABIN5608126

Product Details

Target: IgA

Fragment: F(ab')2 fragment

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: F(ab')2 Human IgA (alpha chain) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against Human IgA, anti-Peroxidase and anti-Goat Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

Characteristics: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Human IgA alpha heavy chain

Isotype: IgG

Application Details

Application Notes: Application Note: This antibody is supplied in 0.01M Sodium Phosphate, 0.25M Sodium Chloride pH : 7.6. F(ab')2 Anti-Human IgA Peroxidase Antibody is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:500 - 1:5,000 Western Blot Dilution: 1:10,000 - 1:200,000 ELISA Dilution: 1:5,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 0.8 mg/mL

Buffer:

Buffer: See application note.

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:None. Do NOT add sodium azide!

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background: F(ab')2 Anti-Human IgA Peroxidase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.