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Goat anti-Rat IgA (Heavy Chain) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
Catalog No. ABIN5608108
  • Target See all IgA products
    IgA
    Binding Specificity
    • 102
    • 61
    • 41
    • 18
    • 15
    • 13
    • 8
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 181
    • 62
    • 41
    • 16
    • 14
    • 13
    • 12
    • 12
    • 11
    • 10
    • 8
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Rat
    Host
    • 200
    • 104
    • 49
    • 17
    • 14
    • 6
    • 2
    Goat
    Clonality
    • 302
    • 63
    Polyclonal
    Conjugate
    • 91
    • 54
    • 49
    • 41
    • 24
    • 19
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 183
    • 131
    • 122
    • 105
    • 101
    • 92
    • 59
    • 47
    • 40
    • 34
    • 28
    • 23
    • 23
    • 18
    • 17
    • 17
    • 16
    • 13
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Characteristics
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Rat IgA alpha heavy chain

    Isotype
    IgG
    Labeling Ratio
    5.0
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IgA
    Abstract
    IgA Products
    Target Type
    Antibody
    Background

    Synonyms: Goat anti-Rat IgA (alpha chain) fluorescein Conjugated Antibody, Goat anti-Rat IgA alpha Antibody FITC Conjugation

    Background: Anti-Rat IgA Fluorescein Antibody generated in goat detects immunoglobulin A heavy chain from rat. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgA is conjugated to Fluorescein.

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