Goat anti-Rat IgA (Heavy Chain) Antibody (FITC) - Preadsorbed
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- Target See all IgA products
- IgA
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Binding Specificity
- Heavy Chain
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Characteristics
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Rat IgA alpha heavy chain
- Isotype
- IgG
- Labeling Ratio
- 5.0
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgA
- Abstract
- IgA Products
- Target Type
- Antibody
- Background
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Synonyms: Goat anti-Rat IgA (alpha chain) fluorescein Conjugated Antibody, Goat anti-Rat IgA alpha Antibody FITC Conjugation
Background: Anti-Rat IgA Fluorescein Antibody generated in goat detects immunoglobulin A heavy chain from rat. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgA is conjugated to Fluorescein.
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