Goat anti-Human IgA (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN5608093

Product Details

Target: IgA

Fragment: F(ab')2 fragment

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgA alpha heavy chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200-1:1,000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:2,000-1:10,000

Western Blot Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Concentration: 0.6 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted powder. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 anti-Human IgA (alpha chain) Antibody alkaline phosphatase Conjugation, Goat F(ab')2 anti-Human IgA alpha alk phos Conjugated Antibody

Background: F(ab')2 Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.