Goat anti-Human IgA (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN5608053

Product Details

Target: IgA

Fragment: F(ab')2 fragment

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Characteristics: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgA alpha heavy chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:5,000

Application Note: F(ab')2 Anti-Human IgA Biotin Antibody is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:20,000 - 1:400,000

Flow Cytometry Dilution: 1:200 - 1:1,000

Western Blot Dilution: 1:2,000 - 1:10,000

Other Performance Data: This product has been overfilled to ensure total recovery of stated quantity.

Comment:

This product has been overfilled to ensure total recovery of stated quantity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 anti-Human IgA (alpha chain) Antibody biotin Conjugation, Goat F(ab')2 anti-Human IgA alpha biotin Conjugated Antibody

Background: F(ab')2 Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.