Rabbit anti-Rat IgG2ab, Subclass Specific Antibody
ID, IEP
Polyclonal
IgG
unconjugated
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- Target See all IgG2ab products
- IgG2ab (IgG2ab, Subclass Specific)
- Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- Specificity
- The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b.
- Characteristics
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre not less then 1:64 when tested against pooled normal rat serum in agar block immunodiff
- Purification
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Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - Immunogen
- Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a and/or IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2a/IgG2b from serum or plasma.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgG2ab (IgG2ab, Subclass Specific)
- Alternative Name
- IgG2ab (IgG2ab Products)
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