Goat anti-Rat IgG (Heavy & Light Chain) Antibody
ICC, ID, IEP, RID
Polyclonal
IgG
unconjugated
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Application
- Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- Specificity
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
- Characteristics
- Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal rat serum in agar block titration.
- Purification
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Delipidated, heat inactivated lyophilized stable whole serum
No adsorption required. - Immunogen
- Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal rat serum, to prepare an immunoadsorbent for the production of Ig-free rat serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water.
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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