Goat anti-Mouse IgG2 Antibody - Preadsorbed

Catalog No. ABIN458515

Product Details

Target: IgG2

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The reactivity of the antiserum is directed to the subclass IgG2a and IgG2b as tested against mouse sera and a panel of purified homogenous immunoglobulins.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As unlabelled primary or secondary reagent for indirect detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10.000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG2

Abstract: IgG2 Products

Target Type: Antibody