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Goat anti-Mouse IgG2 Antibody (TRITC) - Preadsorbed

IHC, ICC, IF Polyclonal IgG TRITC
Catalog No. ABIN458405
  • Target See all IgG2 products
    IgG2
    Reactivity
    • 22
    • 12
    • 7
    • 7
    • 6
    • 1
    Mouse
    Host
    • 19
    • 10
    • 10
    • 10
    • 6
    Goat
    Clonality
    • 30
    • 25
    Polyclonal
    Conjugate
    • 21
    • 8
    • 8
    • 8
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    TRITC
    Application
    • 29
    • 28
    • 24
    • 20
    • 14
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    This renders it specific for use in test systems containing material of human origin (e. g. human tissue/mouse monoclonal antibody to a human tissue constituent/anti mouse Ig isotype-specific immunoconjugate.
    Purification
    Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
    Immunogen
    Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    In immunocytochemical and immunohistochemical single and double staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, as indicating second antibody conjugate to analyze or separate human cells after reacting with monoclonal mouse antibodies in immunofluorescence microscopy, fluorescence activated cell sorting and flow cytometry, to identify and measure IgG2 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    Concentration
    10 mg/mL
    Buffer
    TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Handling Advice
    Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgG2
    Abstract
    IgG2 Products
    Target Type
    Antibody
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