Goat anti-Mouse IgM (Fc Region) Antibody (FITC)

Catalog No. ABIN458370

Product Details

Target: IgM

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgM, Fc specific.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this conjugate has not been tested in detail, however this conjugate has been passed over appropriate immunoadsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate.

Characteristics: The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No reaction is obtained with purified IgA, IgG and IgG/Fab fragments. In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates, to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogen: Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split (7S) IgM. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody