Sheep anti-Human IgG3 Antibody
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- Target See all IgG3 products
- IgG3
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Reactivity
- Human
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Host
- Sheep
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Clonality
- Polyclonal
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Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- Characteristics
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The activity of the antiserum is directed to human IgG3 subclass.
Precipitating polyclonal sheep antisera to human IgG3, subclass specific
Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: The antigenic differences between subclasses are considerably less pronounced than those between classes or betw - Purification
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Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorption with insolubilized highly purified myeloma proteins of the other subclass or other serum proteins as required. - Immunogen
- Purified homogenous IgG3 is isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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Mouse anti-Human IgG3 (Hinge Region) antibody
ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 unconjugated
Mouse anti-Human IgG3 (Hinge Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Monoclonal HP6050 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
Mouse anti-Human IgG3 (Hinge Region) antibody (HRP)ELISA, WB, IHC Monoclonal HP6050 IgG1 HRP
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- Application Notes
- In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG3 subclass in serum or other body fluids. Human IgG constitutes about 75 % of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water.
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgG3
- Abstract
- IgG3 Products
- Target Type
- Antibody
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