Mouse anti-Human IgA2 (IgA2(m)2) Antibody (Biotin)

Catalog No. ABIN457865

Product Details

Target: IgA2

Binding Specificity: IgA2(m)2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Specificity: Biotin-conjugated purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific

Cross-Reactivity (Details): The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Characteristics: The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA2(m)2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assayrelated characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:200 upwards.

Purification: Purified

Immunogen: Highly purified monoclonal IgA2(m)2 isolated from human serum.

Clone: NI 194-3 (A89-040)

Isotype: IgG1

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgA2

Alternative Name: IgA22 (IgA2 Products)