Rabbit anti-Goat IgM (Heavy & Light Chain) Antibody

Catalog No. ABIN457734

Product Details

Target: IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgM molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.

Characteristics: Antibody titre: Precipitin titre not less than 1:64 when tested against normal goat serum in agar block titration.

Purification: No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Purified normal IgM isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C

Storage Comment: The lyophilized IgM fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody