Mouse anti-Human IgE Antibody (Biotin)
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- Target See all IgE products
- IgE
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
- Specificity
- Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
- Sensitivity
- Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone Le27), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)
- Characteristics
- IGHE, chromosome 14q32.33
- Purification
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
- Purity
- > 95 %, tested by analytical Gel Permeation Chromatography
- Sterility
- Sterile filtered
- Immunogen
- purified human myeloma IgE
- Clone
- Le27-NBS01
- Isotype
- IgG1 kappa
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- Application Notes
- For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS, pH=7.2, sterile filtered, contains no NaN3
- Preservative
- Azide free
- Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
- Storage
- 4 °C
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- Target
- IgE
- Abstract
- IgE Products
- Background
- The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
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