Mouse anti-Human IgM Antibody (HRP)
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- Target See all IgM products
- IgM
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- HRP
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Application
- ELISA, Western Blotting (WB)
- Specificity
- The antibody CH2 reacts with Fc fragment of human IgM.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
- Immunogen
- Purified human IgM.
- Clone
- CH2
- Isotype
- IgG1
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- Application Notes
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Western blotting: Recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular and intracellular staining. - Comment
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The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
- Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.,immunoglobulin M
- Molecular Weight
- 900 kDa
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