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- Target See all IgM products
- IgM
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Reactivity
- Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Cross-Reactivity (Details)
- Species reactivity (tested):Porcine.Minimal Cross Reaction with Rat, Goat, Sheep, Donkey, Human (all <0.1 %) and Rabbit (0.1 %).
- Purification
- Affinity Chromatography
- Immunogen
- Porcine IgM.
- Clone
- 206-2
- Isotype
- IgG2b
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- Application Notes
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ELISA: 0.1 μg/mL (1/4000). Immunoprecipitation. Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/2000). Immunohistochemistry on Paraffin Sections (Does not react on routinely processedParaffin Section). Does not react in Western Blot with reduced and denatured samples.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water to make stock solution.
- Concentration
- 0.4 mg/mL
- Buffer
- PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated thawing and freezing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store lyophilized product at 2-8 °C. Store stock solution or aliquots thereof at -28 °C.
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
- IgM antibodies form a major proportion of the primary antibody response, whereas the secondary response consists almost entirely of IgG. IgMs are often referred to as macroglobulins because of their high molecular weight. They form a pentameric structure consisting of five subunits and a single J-chain. Each subunit contains the well-known pairs of heavy and light chains, with the particular feature of the heavy (μ) chain containing one variable and four constant regions. The molecular weight of intact IgM is approximately 900 kDa. In polyacrylamide gel electrophoresis under denaturing conditions, the heavy chain migrates as a band of approximately 85 kDa.Synonyms: Pig Immunoglobulin M
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