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Goat anti-Human IgA Antibody (PE)

ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Catalog No. ABIN376514
  • Target See all IgA products
    IgA
    Fragment
    F(ab')2 fragment
    Reactivity
    • 180
    • 62
    • 42
    • 16
    • 14
    • 13
    • 12
    • 12
    • 11
    • 10
    • 8
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 200
    • 104
    • 49
    • 17
    • 14
    • 6
    • 2
    Goat
    Clonality
    • 302
    • 63
    Polyclonal
    Conjugate
    • 91
    • 55
    • 49
    • 41
    • 24
    • 19
    • 9
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    PE
    Application
    • 182
    • 131
    • 122
    • 105
    • 101
    • 92
    • 59
    • 47
    • 40
    • 34
    • 28
    • 23
    • 23
    • 18
    • 18
    • 17
    • 17
    • 13
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS)
    Specificity
    Reacts with the heavy chain of human IgA
    Characteristics
    Goat F(ab')2 Anti-Human IgA-PE
    Purification
    Affinity chromatography on sheep IgG covalently coupled to agarose. Gel filtration chromatography of pepsin digested antibody.
    Isotype
    IgG
  • Application Notes
    • Applications: Quality tested applications include - ELISA , FLISA FC ,
    • Other referenced applications include - ELISPOT , IHC-FS , Stim
    • Working Dilutions: ELISA BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:100 - 1:400 AF555 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Restrictions
    For Research Use only
  • Buffer
    0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." in: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).

    Magri, Comerma, Pybus, Sintes, Lligé, Segura-Garzón, Bascones, Yeste, Grasset, Gutzeit, Uzzan, Ramanujam, van Zelm, Albero-González, Vazquez, Iglesias, Serrano, Márquez, Mercade, Mehandru, Cerutti: "Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals." in: Immunity, Vol. 47, Issue 1, pp. 118-134.e8, (2017) (PubMed).

  • Target
    IgA
    Abstract
    IgA Products
    Target Type
    Antibody
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