Goat anti-Mouse IgG1 Antibody (PE) - Preadsorbed

Catalog No. ABIN376449

Product Details

Target: IgG1

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy chain of mouse IgG1

Characteristics: Goat Anti-Mouse IgG1, Human ads-PE

Purification:

Purification Method: Affinity chromatography on mouse IgG1 covalently linked to agarose.

Preadsorption: Human Adsorbed

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , IHC-WM , ICC , EM , WB , Multiplex , SPR
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, CY5, APC/CY7, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

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Gaggero, Bruschi, Petretto, Parodi, Del Zotto, Lavarello, Prato, Santucci, Barbuto, Bottino, Candiano, Moretta, Vitale, Moretta, Cantoni: "Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor." in: Oncoimmunology, Vol. 7, Issue 9, pp. e1470730, (2018) (PubMed).

Ratiu, Racine, Hasham, Wang, Branca, Chapman, Zhu, Donghia, Philip, Schott, Wasserfall, Atkinson, Mills, Leeth, Serreze: "Genetic and Small Molecule Disruption of the AID/RAD51 Axis Similarly Protects Nonobese Diabetic Mice from Type 1 Diabetes through Expansion of Regulatory B Lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 11, pp. 4255-4267, (2017) (PubMed).

Priglinger, Szober, Priglinger, Merl, Euler, Kernt, Gondi, Behler, Geerlof, Kampik, Ueffing, Hauck: "Galectin-3 Induces Clustering of CD147 and Integrin-β1 Transmembrane Glycoprotein Receptors on the RPE Cell Surface." in: PLoS ONE, Vol. 8, Issue 7, pp. e70011, (2013) (PubMed).

Hasham, Snow, Donghia, Branca, Lessard, Stavnezer, Shopland, Mills: "Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2374-82, (2012) (PubMed).

Volná, Jarjour, Baxter, Roffler, Monnat, Stoddard, Scharenberg: "Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases." in: Nucleic acids research, Vol. 35, Issue 8, pp. 2748-58, (2007) (PubMed).

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody