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Goat anti-Mouse IgG1 (Heavy Chain) Antibody (HRP)

Name: Goat anti-Mouse IgG1 (Heavy Chain) Antibody (HRP)
Brand: antibodies-online
SKU: ABIN376248
Price: 247.2 USD
Availability: InStock

ELISA, WB, IHC Polyclonal IgG HRP

Lane 1 - DEC Monoclonal AntibodyLane 2 - DEC-HER2 Monoclonal AntibodyLane 3 - Control Ig-HER2 Monoclonal AntibodyHER2 extracellular domain was cloned in frame into the COOH terminus of anti-DEC (DEC-HER2). (Goat anti-Mouse IgG1 (Heavy Chain) Antibody (HRP))

1 image

(2 references)

Catalog No. ABIN376248

Datasheet as PDF

Target See all IgG1 products

IgG1
Binding Specificity
51

26

17

6

5

3

3

2

2

1

1

1

1

1
Heavy Chain
Reactivity
95

24

23

7

7

5

4

4

3

2

1
Mouse
Host
62

42

37

14

10

6

1
Goat
Clonality
94

57
Polyclonal
Conjugate
46

20

19

18

11

8

7

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
HRP
Application
84

58

53

45

32

21

18

15

11

10

10

9

5

4

3

2

2

2
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity

Reacts with the heavy chain of mouse IgG1 as demonstrated by ELISA and flow cytometry.

Characteristics

Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry to conform to characteristics of a standard reference reagent.

Purification

Affinity chromatography on mouse IgG1 covalently linked to agarose. Cross Absorption: Mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.

Isotype

IgG
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody - Preadsorbed
ELISA, WB, IHC Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Rat anti-Mouse IgG1 antibody
ELISA, FACS Monoclonal SB77e IgG2b unconjugated

Rat anti-Mouse IgG1 antibody (HRP)
ELISA Monoclonal SB77E IgG2b HRP

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Atto 647N) - Preadsorbed
WB, FACS, FLISA, FM Polyclonal IgG Atto 647N

Application Notes

Working Dilution:
ELISA: 1:4,000-1:8,000
Indirect immunostaining in conjunction with mouse IgG1 monoclonal antibodies.
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions

For Research Use only
Format

Liquid

Handling Advice

Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage

4 °C/-20 °C

Storage Comment

Store at 2-8 °C or long-term at -20 °C.
Margry, Kersemakers, Hoek, Arkesteijn, Wieland, van Eden, Broere: "Activated peritoneal cavity B-1a cells possess regulatory B cell properties." in: PLoS ONE, Vol. 9, Issue 2, pp. e88869, (2014) (PubMed).

Stegmann, Kamphuis, Meijerhof, Goud, de Haan, Wilschut: "Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation." in: Vaccine, (2010) (PubMed).
Target

IgG1

Abstract

IgG1 Products

Target Type

Antibody