Goat anti-Human lambda (Chain lambda) Antibody (Biotin)

Catalog No. ABIN375963

Product Details

Target: lambda

Fragment: F(ab')2 fragment

Binding Specificity: Chain lambda

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), ELISA

Specificity: Reacts with humanκ light chains as demonstrated by ELISA and flow cytometry.

Cross-Reactivity (Details): Cross Absorption: Pooled human myeloma proteins withκ light chains and mouse immunoglobulins.

Characteristics: Goat F(ab')2 Anti-Human Lambda-BIOT

Purification: Affinity chromatography on pooled human Igs with λ light chains covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA FLISA FC ,
• Other referenced applications include - WB , Stim
• Working Dilutions: ELISA HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

B cell enumeration

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: lambda