Goat anti-Human IgM Antibody (Biotin)

Catalog No. ABIN375949

Product Details

Target: IgM

Fragment: F(ab')2 fragment

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy chain of human IgM

Characteristics: Goat F(ab')2 Anti-Human IgM-BIOT

Purification: Affinity chromatography on pooled human Igs with light chains covalently linked to agarose

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ICC , ELISPOT , Stim
• Working Dilutions: ELISA HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

White, Pone, Lam, Tat, Hayama, Li, Zan, Casali: "Histone Deacetylase Inhibitors Upregulate B Cell microRNAs That Silence AID and Blimp-1 Expression for Epigenetic Modulation of Antibody and Autoantibody Responses." in: Journal of immunology (Baltimore, Md. : 1950), (2014) (PubMed).

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody