Mouse anti-Human IgG (Fc Region) Antibody (APC)

Catalog No. ABIN375810

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: APC

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA,

Characteristics: Mouse Anti-Human IgG Fc-APC

Purification: Purified

Immunogen: Unknown

Clone: H2

Isotype: IgG2b

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , ELISPOT - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:8,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:10,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:600 AF488 and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells CY5 conjugate 0.3 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Eavarone, Al-Alem, Lugovskoy, Prendergast, Nazer, Stein, Dransfield, Behrens, Rueda: "Humanized anti-Sialyl-Tn antibodies for the treatment of ovarian carcinoma." in: PLoS ONE, Vol. 13, Issue 7, pp. e0201314, (2019) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody