Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (APC) - Preadsorbed

Catalog No. ABIN375766

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: APC

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA

Characteristics: Goat Anti-Mouse IgG(H+L), Human ads-APC

Purification:

Purification Method: Affinity chromatography on mouse IgG covalently linked to agarose.

Preadsorption: Human Adsorbed

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA , FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, CY5, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Yousefi, Günther, Hörner, Chalupsky, Wess, Brandl, Smith, Fleck, Kunkel, Zurbriggen, Höfer, Weber, Schamel: "Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor." in: eLife, Vol. 8, (2019) (PubMed).

Prendergast, Galvao da Silva, Eavarone, Ghaderi, Zhang, Brady, Wicks, DeSander, Behrens, Rueda: "Novel anti-Sialyl-Tn monoclonal antibodies and antibody-drug conjugates demonstrate tumor specificity and anti-tumor activity." in: mAbs, Vol. 9, Issue 4, pp. 615-627, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody