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Goat anti-Mouse IgM Antibody (APC) - Preadsorbed

ELISA, FACS Polyclonal IgG APC
Catalog No. ABIN375765
  • Target See all IgM products
    IgM
    Reactivity
    • 225
    • 155
    • 88
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    Mouse
    Host
    • 364
    • 231
    • 85
    • 18
    • 14
    • 5
    • 4
    • 1
    • 1
    Goat
    Clonality
    • 533
    • 88
    Polyclonal
    Conjugate
    • 121
    • 86
    • 84
    • 65
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    APC
    Application
    • 374
    • 280
    • 261
    • 221
    • 130
    • 124
    • 91
    • 91
    • 64
    • 62
    • 53
    • 52
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS)
    Specificity
    Reacts with the heavy chain of mouse IgM
    Characteristics
    Goat Anti-Mouse IgM, Human ads-APC
    Purification

    Purification Method: Affinity chromatography on mouse IgM covalently linked to agarose.

    Preadsorption: Human Adsorbed

    Isotype
    IgG
  • Application Notes
    • Applications: Quality tested applications include - ELISA , FLISA FC ,
    • Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , EM , WB , IP , Purification , Stim
    • Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, CY5, PE/CY7, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Comment

    Excitation/Emission wavelength: 650 nm/660 nm

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Howie, Delaney, Wang, Er, Vidarsson, Stegmann, Kapp, Lebedev, Wu, AuBuchon, Zimring: "Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent." in: Transfusion, Vol. 56, Issue 12, pp. 2953-2962, (2017) (PubMed).

  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
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