Goat anti-Mouse IgM Antibody (APC) - Preadsorbed
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- Target See all IgM products
- IgM
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- APC
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Application
- ELISA, Flow Cytometry (FACS)
- Specificity
- Reacts with the heavy chain of mouse IgM
- Characteristics
- Goat Anti-Mouse IgM, Human ads-APC
- Purification
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Purification Method: Affinity chromatography on mouse IgM covalently linked to agarose.
Preadsorption: Human Adsorbed
- Isotype
- IgG
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- Application Notes
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- Applications: Quality tested applications include - ELISA , FLISA FC ,
- Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , EM , WB , IP , Purification , Stim
- Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, CY5, PE/CY7, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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Excitation/Emission wavelength: 650 nm/660 nm
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent." in: Transfusion, Vol. 56, Issue 12, pp. 2953-2962, (2017) (PubMed).
: "
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Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent." in: Transfusion, Vol. 56, Issue 12, pp. 2953-2962, (2017) (PubMed).
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
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