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Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Name: Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
Brand: antibodies-online
SKU: ABIN375738
Price: 264 USD
Availability: InStock

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-AF488 and DAPI. (Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)))

1 image

Catalog No. ABIN375738

Datasheet as PDF

Target See all IgG products

IgG
Binding Specificity
2381

512

424

207

132

129

95

29

28

28

22

9

6

6

6

6

5

2

2

1

1
Heavy & Light Chain
Reactivity
724

699

602

476

403

243

212

176

147

138

113

112

107

102

82

59

47

28

28

16

12

10

8

5

5

5

3

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Rat
Host
1778

1576

571

241

112

111

33

31

21

19

15

11

11

1
Donkey
Clonality
3434

175

1
Polyclonal
Conjugate
588

417

416

381

281

276

244

118

108

67

66

64

61

58

52

51

48

44

44

44

41

39

35

32

31

31

30

29

29

29

29

29

29

28

28

28

28

28

28

28

27

27

26

26

25

22

21

19

17

17

16
Alkaline Phosphatase (AP)
Application
1952

1838

1806

1519

934

910

652

478

380

236

236

234

106

103

86

67

53

52

31

28

25

21

18

16

11

9

4

4

4

4

3

2

2

2

2

1

1

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity

Reacts with the heavy and light chains of Rat IgG as demonstrated by ELISA.

Characteristics

Source: Pooled antisera from donkey hyperimmunized with rat IgG.
To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA for conformance to characteristics of a standard reference reagent.

Purification

Affinity chromatography on mouse IgG covalently linked to agarose.

Isotype

IgG
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 488

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 647N

Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Rat IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 425) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 425

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 532) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 532

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 594) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 594

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Biotin

Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Application Notes

Working Dilution:
1:2,000-1:4,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions

For Research Use only
Format

Liquid

Handling Advice

Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage

4 °C
Target

IgG

Abstract

IgG Products

Target Type

Antibody