Mouse anti-Human IgE (Fc Region) Antibody
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- Target See all IgE products
- IgE
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Binding Specificity
- Fc Region
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
- Specificity
- Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
- Characteristics
- IGHE, chromosome 14q32.33
- Purification
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
- Purity
- > 95 %, tested by analytical Gel Permeation Chromatography
- Sterility
- Sterile filtered
- Immunogen
- purified human myeloma IgE
- Clone
- BSW17-NBS01
- Isotype
- IgG1 kappa
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- Application Notes
- For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS, pH=7.2, sterile filtered, contains no NaN3
- Preservative
- Azide free
- Handling Advice
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
- Storage
- 4 °C
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Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
: "
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Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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- Target
- IgE
- Abstract
- IgE Products
- Background
- The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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