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Goat anti-Human IgA Antibody (Alkaline Phosphatase (AP))

ELISA, IHC, WB, ICC, IA Polyclonal Alkaline Phosphatase (AP)

Catalog No. ABIN336539

Datasheet as PDF

Target See all IgA products

IgA
Reactivity
180

62

42

16

14

13

12

12

11

10

8

6

6

5

4

3

3

2

1

1
Human
Host
200

104

49

17

14

6

2
Goat
Clonality
302

63
Polyclonal
Conjugate
91

55

49

41

24

18

9

6

6

6

6

5

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Alkaline Phosphatase (AP)
Application
182

130

121

106

100

92

59

47

40

34

28

23

23

18

18

17

16

13

7

7

4

3

2

2

2

2

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)

Purification

Affinity purified using solid phase Human IgA

Immunogen

Goat serum was obtained from animals of US origin and under the care of a registered veterinarian.
Rabbit anti-Human IgA antibody
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated

Goat anti-Human IgA antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Human IgA antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Human IgA (Heavy Chain) antibody (Biotin)
ELISA, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgA (Heavy Chain) antibody (FITC)
ELISA, FACS Polyclonal IgG FITC

Goat anti-Human IgA (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgA antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgA antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human IgA (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Goat anti-Human IgA (Heavy Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Biotin

Application Notes

This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry

Comment

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions

For Research Use only
Buffer

30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05 % (w/v) Sodium Azide

Preservative

Sodium azide

Precaution of Use

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice

Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage

4 °C
Target

IgA

Abstract

IgA Products

Target Type

Antibody