Goat anti-Human IgE Antibody (Alkaline Phosphatase (AP))
WB, EIA
Polyclonal
IgG
Alkaline Phosphatase (AP)
-
- Target See all IgE products
- IgE
-
Reactivity
- Human, Monkey
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- Alkaline Phosphatase (AP)
-
Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Cross-Reactivity
- Monkey
- Purification
- Purified IgG prepared by affinity chromatography
- Isotype
- IgG
-
-
- Application Notes
-
ELISA: 1/1000-1/10000. Western Blot: 1/1000-1/5000.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- 50 mM HEPES containing 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
-
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
-
- Target
- IgE
- Abstract
- IgE Products
- Background
- Synonyms: Immunoglobulin E human
-
You are here:
