Mouse anti-Human IgG Fc (Fc Region) Antibody (HRP)
ELISA, WB
Monoclonal
EM-07
IgG1
HRP
-
- Target
- IgG Fc
- Binding Specificity
- Fc Region
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- HRP
-
Application
- ELISA, Western Blotting (WB)
- Specificity
- The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
- Cross-Reactivity (Details)
- Human, Other not determined
- Purification
- Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
- Immunogen
- Fusion protein of human IgG Fc fragment.
- Clone
- EM-07
- Isotype
- IgG1
-
Mouse anti-Human IgG Fc (Fc Region) antibody
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated
-
- Application Notes
- Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
- Comment
-
The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
-
- Target
- IgG Fc
- Alternative Name
- IgG (Fc)
- Target Type
- Antibody
- Background
- Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment
- Molecular Weight
- 150 kDa
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