Donkey anti-Rat IgG (Whole Molecule) Antibody (Biotin)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Whole Molecule
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Reactivity
- Rat
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep serum proteins.
- Purification
- Immunoaffinity purified
- Immunogen
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Bovine IgG whole molecule.
Type of Immunogen: Purified protein - Isotype
- IgG
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- Application Notes
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Approved: ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:2000), WB (1:1000 - 1:5000)
Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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