Rabbit anti-Mouse IgG Antibody (Biotin)
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- Target See all IgG products
- IgG
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against other Mouse heavy or light chain proteins
- Purification
- Immunoaffinity purified
- Immunogen
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Mouse IgG gamma chain.
Type of Immunogen: Purified protein
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- Application Notes
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Approved: ELISA (1:10000 - 1:50000), IF, IHC, WB
Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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