Goat anti-Rabbit IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN2669853

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Lateral Flow (LF)

Specificity: IgG F(ab')2

Cross-Reactivity: Rabbit

Characteristics: Anti-Rabbit antibody generated in goat detects specifically rabbit IgG F(s). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG f(c) fragment in goat

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Antibody Anti-Rabbit IgG F(c) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.99 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Rabbit IgG F(c) Antibody, Gt-a-Rabbit IgG F(c), Rabbit IgG F(c) Antibody in goat, Goat IgG F(c) Secondary Antibody.

Background: Anti-Rabbit IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.