Mouse anti-Human IgG (Fc fragment) Antibody
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc fragment
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- ELISA, Western Blotting (WB)
- Characteristics
- Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
- Purification
- Antibody is purified by protein A affinity chromatography.
- Immunogen
- Chrompure Human IgG, Fc Fragment.
- Clone
- 4D2D9G8
- Isotype
- IgG1
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- Application Notes
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Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test. - Restrictions
- For Research Use only
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- Buffer
- Purified antibody in PBS containing 0.03 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Storage
- -20 °C
- Storage Comment
- IgG monoclonal antibody can be stored at -20 °C, stable for one year.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Gene ID
- 2212
- UniProt
- P12318
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