Donkey anti-Rabbit IgG Antibody (AbBy Fluor® 555)

Catalog No. ABIN2176495

Product Details

Target: IgG

Reactivity: Rabbit

Host: Donkey

Clonality: Polyclonal

Conjugate: AbBy Fluor® 555

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Purification: Purified by Protein A.

Isotype: IgG

Application Details

Application Notes: IF(IHC-P): (1:500-2000), IF(IHC-F): (1:500-2000), IF(ICC): (1:500-1000)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4 °C for 12 months.

References

Kashiwabara, Ambe, Nakagawa, Watanabe: "Immunohistochemical localization of Nox in mouse circumvallate papillae." in: Tissue & cell, Vol. 47, Issue 6, pp. 550-8, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.