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Goat anti-Human IgA (Chain alpha) Antibody (Biotin) - Preadsorbed

WB, IHC (fro), EIA Polyclonal Biotin
Catalog No. ABIN181418
  • Target See all IgA products
    IgA
    Binding Specificity
    • 103
    • 61
    • 40
    • 18
    • 15
    • 13
    • 8
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Chain alpha
    Reactivity
    • 180
    • 62
    • 42
    • 16
    • 14
    • 13
    • 12
    • 12
    • 11
    • 10
    • 8
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 200
    • 104
    • 49
    • 17
    • 14
    • 6
    • 2
    Goat
    Clonality
    • 302
    • 63
    Polyclonal
    Conjugate
    • 91
    • 55
    • 49
    • 40
    • 24
    • 19
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 183
    • 130
    • 122
    • 106
    • 101
    • 92
    • 58
    • 47
    • 40
    • 34
    • 27
    • 23
    • 23
    • 18
    • 18
    • 17
    • 17
    • 13
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Characteristics
    Molar Ratio: 10-20 BAC molecules per Goat IgG molecule
    Purification
    Preadsorption: Solid phase absorption
    Immunogen
    Human IgA (alpha chain)
  • Application Notes
    Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibodybased assays using streptavidin or avidin conjugates: ELISA: 1/60,000-1/600,000
    Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1.0 mL of deionized water
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containg 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid cycles of freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 °C prior to restoration. For extended storage after reconstitution, mix product with glycerol to 50 % final, then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.
  • Target
    IgA
    Abstract
    IgA Products
    Target Type
    Antibody
    Background
    Synonyms: Human Immunoglobulin A
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