Goat anti-Human IgA (Chain alpha) Antibody (Biotin) - Preadsorbed
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- Target See all IgA products
- IgA
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Binding Specificity
- Chain alpha
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Characteristics
- Molar Ratio: 10-20 BAC molecules per Goat IgG molecule
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Human IgA (alpha chain)
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- Application Notes
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Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibodybased assays using streptavidin or avidin conjugates: ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containg 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage after reconstitution, mix product with glycerol to 50 % final, then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- IgA
- Abstract
- IgA Products
- Target Type
- Antibody
- Background
- Synonyms: Human Immunoglobulin A
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