Goat anti-Mouse Ig Antibody (CypHer5E)
ICC
Polyclonal
CypHer5E
Fab fragment
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- Target See all Ig products
- Ig
- Fragment
- Fab fragment
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- CypHer5E
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Application
- Immunocytochemistry (ICC)
- Purification
- affinity purified Fab fragments. Rabbit serum albumin was added for stabilization.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. Application Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µL for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay. ICC: image IMPORTANT NOTE FOR NON-EUROPEAN CUSTOMERS: Please provide us with your import permit for goat-derived biological reagents when ordering this product!
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives.
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- Target
- Ig
- Abstract
- Ig Products
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