Mouse anti-Human IgM (Fc5mu Region) Antibody

Catalog No. ABIN1607857

Product Details

Target: IgM

Binding Specificity: Fc5mu Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Specificity: IgM Fc5µ

Cross-Reactivity: Human

Purification: Anti-Human IgM Fc5μ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5μ.  No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.

Immunogen:

Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in mice.

Immunogen Type: Native Protein

Clone: 22G1-H8

Isotype: IgG2b kappa

Application Details

Application Notes:

Application Note: Human IgM Fc5μ antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:1000-1:10000

Western Blot Dilution: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: mouse anti-Human IgM Fc5μ Antibody, Human IgM Fc5u Antibody, Ms-a-Human IgM Fc5μ Antibody, Human IgM Fc5μ Antibody in Mouse, Human M Fc5μ Secondary Antibody

Background: Anti-Human IgM Fc5μ antibody generated in mice specifically detects Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.