Mouse anti-Human IgG3 (Hinge Region) Antibody

Catalog No. ABIN135664

Product Details

Target: IgG3

Binding Specificity: Hinge Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Reacts with the hinge region of the heavy chain of human IgG3 as demonstrated by ELISA

Characteristics: Mouse Anti-Human IgG3 Hinge-UNLB

Purification: Purified

Immunogen: Human IgG3 myeloma protein

Clone: HP6050

Isotype: IgG1

Application Details

Application Notes: Working Dilution:
Immunofluorescence: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.

Handling Advice: Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

References

Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

Haag, Schneider, Mason, Tuncel, Andersson, Peters, Burkhardt, Holmdahl: "Mass spectrometric analysis of citrullinated type II collagen reveals new citrulline-specific autoantibodies, which bind to human arthritic cartilage." in: Arthritis & rheumatology (Hoboken, N.J.), (2014) (PubMed).

Jackson, Lucas, Melancon, Desai: "Clinical relevance and IgG subclass determination of non-HLA antibodies identified using endothelial cell precursors isolated from donor blood." in: Transplantation, Vol. 92, Issue 1, pp. 54-60, (2011) (PubMed).

Xu, Chen, Zhao: "High potential to reverse the inhibition of myeloperoxidase by ceruloplasmin of anti-myeloperoxidase autoantibodies of IgG3 subclass." in: Autoimmunity, (2011) (PubMed).

Xu, Chen, Cui, Zhao: "Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis." in: Rheumatology (Oxford, England), Vol. 49, Issue 11, pp. 2068-75, (2010) (PubMed).

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody