Mouse anti-Human IgG1 (Hinge Region) Antibody (Biotin)
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- Target See all IgG1 products
- IgG1
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Binding Specificity
- Hinge Region
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- ELISA
- Specificity
- Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA
- Characteristics
- Mouse Anti-Human IgG1 Hinge-BIOT
- Purification
- Protein A affinity column
- Immunogen
- Unknown
- Clone
- 4E3
- Isotype
- IgG1
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- Application Notes
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- Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Purification - Reported in literature , Note - For direct immunostaining of IgG, positive cells in flow cytometry applications, clone HP, (SB Cat. No. ,) is recommended
- Working Dilutions: ELISA Purified (UNLB) antibody ≤ 5 g/mL AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg in 1.0 mL of PBS/Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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- Target
- IgG1
- Abstract
- IgG1 Products
- Target Type
- Antibody
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