Mouse anti-Human IgG1 (Hinge Region) Antibody (Alkaline Phosphatase (AP))
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- Target See all IgG1 products
- IgG1
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Binding Specificity
- Hinge Region
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA
- Specificity
- Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA
- Purification
- Immunoaffinity chromatography
- Clone
- 4E3
- Isotype
- IgG1
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- Application Notes
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Working Dilution:
ELISA: 1:500-1:1,000
Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 1.0 mL of stock solution in 50 mM Tris / 0 mM MgCl2 / 50 % Glycerol, pH 8.0, containing 0.1 % NaN3 as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
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- Target
- IgG1
- Abstract
- IgG1 Products
- Target Type
- Antibody
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