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Mouse anti-Human IgG1 (Hinge Region) Antibody (FITC)

ELISA Monoclonal 4E3 IgG1 FITC
Catalog No. ABIN135623
  • Target See all IgG1 products
    IgG1
    Binding Specificity
    • 52
    • 26
    • 17
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Hinge Region
    Reactivity
    • 96
    • 23
    • 23
    • 7
    • 7
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    Human
    Host
    • 63
    • 42
    • 36
    • 14
    • 10
    • 6
    • 1
    Mouse
    Clonality
    • 95
    • 56
    Monoclonal
    Conjugate
    • 46
    • 20
    • 19
    • 18
    • 11
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 84
    • 59
    • 53
    • 46
    • 32
    • 21
    • 18
    • 15
    • 11
    • 10
    • 10
    • 9
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    ELISA
    Specificity
    Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA
    Characteristics
    Mouse Anti-Human IgG1 Hinge-FITC
    Purification
    Immunoaffinity chromatography
    Immunogen
    Unknown
    Clone
    4E3
    Isotype
    IgG1
  • Application Notes
    • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Purification - Reported in literature , Note - For direct immunostaining of IgG, positive cells in flow cytometry applications, clone HP, (SB Cat. No. ,) is recommended
    • Working Dilutions: ELISA Purified (UNLB) antibody ≤ 5 g/mL AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg in 1.0 mL of PBS/Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Dilute only prior to immediate use
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Wang, Qiu, Wang, Zhao, Jin, Xu, Rong, Ge, Zhang, Wang, Zhu: "Role of HLA-G and NCR in Protection of Umbilical Cord Blood Hematopoietic Stem Cells from NK Cell-mediated Cytotoxicity." in: Journal of cellular and molecular medicine, (2010) (PubMed).

  • Target
    IgG1
    Abstract
    IgG1 Products
    Target Type
    Antibody
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