Goat anti-Mouse IgG, IgM Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
Rockland 610-105-115
ELISA, WB, IHC
Polyclonal
IgG
Alkaline Phosphatase (AP)
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- Target See all IgG, IgM products
- IgG, IgM
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 610-105-115
- Supplier
- Rockland
- Purpose
- Mouse IgG + IgM (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- Cross-Reactivity (Details)
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
- Characteristics
- Anti-Mouse IgG3 Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).
- Purification
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Mouse IgG and IgM whole molecules
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Mouse IgG + IgM Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- IgG, IgM
- Alternative Name
- IgG,IgM (IgG, IgM Products)
- Target Type
- Antibody
- Background
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Alkaline Phosphate Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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