Goat anti-Mouse IgG, IgM Antibody (HRP) - Preadsorbed

Catalog No. ABIN129742

Supplier Product No.: 610-103-115

Product Details

Target: IgG, IgM

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 610-103-115

Supplier: Rockland

Purpose: Mouse IgG + IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against No reaction was observed against bovine, horse or human serum proteins.

Characteristics: Anti-Mouse IgG3 Peroxidase Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Mouse IgG and IgM whole molecule was produced by repeated immunization with Mouse IgG and IgM whole molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Mouse IgG and IgM Peroxidase conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:40,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG, IgM

Alternative Name: IgG,IgM (IgG, IgM Products)

Target Type: Antibody

Background: Anti-Mouse IgG and IgM whole molecule peroxidase conjugated antibody generated in goat detects specifically Mouse IgG and IgM whole molecule. This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.