Goat anti-Mouse IgG, IgM Antibody (FITC) - Preadsorbed
Rockland 610-102-115
WB, DB, FM
Polyclonal
IgG
FITC
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- Target See all IgG, IgM products
- IgG, IgM
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Western Blotting (WB), Dot Blot (DB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-102-115
- Supplier
- Rockland
- Purpose
- Mouse IgG + IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
- Cross-Reactivity (Details)
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
- Characteristics
- Anti-Mouse IgG3 Fluorescein Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).
- Purification
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
- Optional[Immunogen]: Mouse IgG and IgM whole molecules
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Mouse IgG + IgM Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Concentration
- 2.2 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG, IgM
- Alternative Name
- IgG,IgM (IgG, IgM Products)
- Target Type
- Antibody
- Background
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Fluorescein Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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