Mouse anti-Rat IgG2c (Heavy Chain) Antibody
EIA
Monoclonal
MARG2c-5
IgG2a
unconjugated
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- Target See all IgG2c products
- IgG2c
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Binding Specificity
- Heavy Chain
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- Enzyme Immunoassay (EIA)
- Purification
- Affinity Chromatography
- Immunogen
- IgG2c (Rat strain LOU/c). Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- MARG2c-5
- Isotype
- IgG2a
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- Application Notes
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ELISA: 5 μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG2c (SA024). Flow Cytometry: 1/200 - 1/1000, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- Target
- IgG2c
- Abstract
- IgG2c Products
- Target Type
- Antibody
- Background
- Synonyms: Rat Immunoglobulin G2c
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